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. 2015 Jan;21(1):124–134. doi: 10.1261/rna.047282.114

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© 2014 Roque et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Interactions in strains deleted for various domains of Pab1 and eRF3. (A,B) Coimmunoprecipitation assays on yeast extracts from strains SKY1085, SKY1081, and SKY1182 expressing various versions of eRF3 as indicated in A; and BSY1552, BSY1537, and W303-1B expressing various versions of Pab1 as indicated in B using anti-eRF3 polyserum (IP anti-eRF3) or nonspecific anti-serum (IP ns-ab) as a negative control. Positions of the different forms of Pab1 and eRF3 are indicated. Aliquots of extract were analyzed (Input) and represent 1/80 of the quantity loaded in the IP lanes. The star indicates the position of residual signal from IgG. (C) Growth phenotype of the following strains: SKY1263, SKY1264, SKY1265, SKY1266 (upper panel); SKY1317, SKY1318, SKY1319, SKY1320 (lower panel) resulting from crossing strains eRF3ΔNM (SKY1182) with pab1ΔLC (BSY1552) or pab1ΔC (BSY1537), and isolated from representative tetrads. Serial dilutions of overnight liquid cultures were deposited on YPDA plates and their growth was scored after 3 d of growth for 30°C and 37°C, and 4 d for 18°C.