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. 2015 Jan;21(1):93–112. doi: 10.1261/rna.047084.114

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© 2014 Garcia-Moreno et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 8. — Effect of Tg treatment in PKR^−/− MEFs transfected with in vitro-synthesized SINV sgmRNAs. (A) sgmRNA C+luc, sgmRNA C+luc AUG-hp, and sgmRNA C+luc AUG-CAA₁₄ synthesized in vitro by T7 RNA polymerase were transfected into PKR^−/− MEFs. Thirty minutes later, cells were treated with 1.5 μM Tg or left untreated, and incubated for 90 min before harvesting to measure luciferase activity. (B–D) PKR^−/− MEFs were transfected and treated as in A, and then collected in sample buffer. SINV C protein, phosphorylated eIF2α, and total eIF2α were detected by immunoblotting using specific antibodies (left panels). Densitometric quantifications of protein C and mutant proteins C₁ or C₂ in the presence or absence of Tg are shown in the right panels. The results displayed in A–D are the mean ± SD of three independent experiments. The percentage values of Tg-treated cells relative to untreated cells are indicated.