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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Circ Arrhythm Electrophysiol. 2012 Jan 13;5(1):163–172. doi: 10.1161/CIRCEP.111.967604

Figure 5.

Figure 5

Subcellular distribution of Cx40-WT and Cx40-Q58L in transiently transfected cells. HeLa cells were transiently transfected with pEFGPN1-Cx40-WT (3.0 μg; panels A), pCMV-FLAG-Cx40-Q58L (3.0 μg; panels B) or pEFGPN1-Cx40-WT (1.5 μg) plus pCMV-FLAG-Cx40-Q58L (1.5 μg; panels C), immunostained for the respective tag protein and visualized by confocal laser scanning microscopy. Notice gap junction plaques (A) absent in Q58L transfectants (B) and present in some (D) but not all (C) co-transfected cells. Bars: 20 μm.