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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Circ Arrhythm Electrophysiol. 2012 Jan 13;5(1):163–172. doi: 10.1161/CIRCEP.111.967604

Figure 6.

Figure 6

Mutant subunit abundance correlated with gap junction function. A: N2A cells were transiently transfected with 3 μg Cx40 constructs in IRES plasmids (see below). Cell lysates were analyzed by Western blot using anti-Cx40 (upper panel) and anti-GAPDH antibodies (lower panel). The number in each lane corresponds to plasmid noted below. Samples from cells co-transfected with plasmids 1 and 2 (1.5 μg each) were loaded on lane 6. Double bands of WT-Cx40 (40 kDa) and Q58L-EGFP (67 kDa) are shown in lanes 3,4, 6,7. Results were repeated in three separate experiments. Overexposure (lane 7) confirmed expression of the high molecular weight protein in lane 3. B: Junctional conductance of heteromeric construct Q58L-IRES-WT (n=15) showed significantly reduced conductance (p<0.001 vs. WT-IRES-WT and WT-IRES-Q58L). *** and NS indicate p<0.001 and no statistical significance, respectively.