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. Author manuscript; available in PMC: 2015 Dec 12.
Published in final edited form as: Curr Protoc Nucleic Acid Chem. 2014 Dec 12;59:7.21.1–7.21.13. doi: 10.1002/0471142700.nc0721s59

Figure 1.

Figure 1

Chemical structures of (A) thymidine (T), (B) O4-methylthymidine (O4MeT) inserts, (C) 5’-(6-carboxyfluorescein)-thymidine ((FAM)T) and (D) sequences of DNA template and primer indicating positions of the modification (X) and (FAM)T.