Table 1.

Stock concentrations, final reaction concentrations and volumes to add for the preparation of individual premixes sufficient for 10 reaction points. Concentrations are corrected for the dilution with the final addition of dNTP

Component	[Stock]	[Reaction]	Volume (μL) to add
DTT (mM)	100	10	11
BSA (mg/mL)	2	0.1	5.5
Glycerol (%)	50	5	11
MgCl2 (mM)	25	5	22
KCl (mM)	2500	100	4.4
Tris-Cl, pH 7.5 (mM)	500	40	8.8
DNA duplex (μM)	200	5	2.75
DNA polymerase (nM)	20	2.5	13.75
H2O	—	—	8.8
Total (μL)			88