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. 2014 Dec 23;34(6):e00168. doi: 10.1042/BSR20140137

Figure 4. Cross-linking of hTS protein with TS1 RNA constructs in which the start codon U95 was substituted by 4-thiouridine.

Figure 4

(A) Denaturing PAGE analysis of cross-linked complexes with TS1-33 and TS1-14 RNA (for RNA sequences see Figure 1). Positions are indicated of the hTS monomer and dimer in the free form (m, d) and cross-linked (×m, ×d). Protein dimer may be present due to incomplete denaturation (see Supplementary Figure S5). (B) Native PAGE analysis of hTS cross-linked with TS1–33 RNA. Both protein and RNA were at 200 μM concentration. Free protein, cross-linked dimer (×d) and monomer (×m) are indicated. Conditions marked by * contain 1% zwitterionic detergent (CHAPS). (C) Native PAGE analysis of a cross-linking time course of hTS protein and TS1–33 RNA. Protein and RNA concentrations were 25 and 10 μM, respectively. Free protein, cross-linked dimer (×d) and monomer (×m) are indicated.