Table 2. Kinetic constants of MmNQO with NADH and NADPH (0.1–150 μM) and different electron acceptors at saturating concentrations.
DCPIP, dichlorophenolindophenol; n.d., not determined (the affinity was too low and no reliable kinetic constants could be determined).
| NADH | Electron acceptor DCPIP | Km (μM) 17.5 | kcat (min−1) 4.95 | kcat/Km (μM−1·min−1) 0.283 |
|---|---|---|---|---|
| Coenzyme Q10 | 33.7 | 6.86 | 0.204 | |
| Potassium ferricyanide | 74.8 | 14.3 | 0.191 | |
| Fc+ | 64.8 | 11.2 | 0.173 | |
| 1,4-BQ | 56.5 | 9.74 | 0.172 | |
| Coenzyme Q1 | 96.8 | 15.6 | 0.161 | |
| Diethyl-oxalacetate sodium salt | 63.2 | 9.73 | 0.154 | |
| Sodium fumarate dibasic | 41.4 | 6.15 | 0.149 | |
| DL-malic acid | 102.5 | 14.2 | 0.139 | |
| Sodium thiosulphate | 257.9 | 19.8 | 0.077 | |
| NADPH | Coenzyme Q1 | 111.6 | 14.6 | 0.130 |
| 1,4-BQ | 48.1 | 6.0 | 0.125 | |
| Potassium ferricyanide | n.d. | n.d. | n.d. |