Table 1. Thermodynamic parameters for acyl-CoA binding to rAtACBP6.
ITC experiments were carried out at 30°C in 10 mM sodium phosphate buffer (pH 7.0). The values are the means of three experiments±S.D.
| Acyl-CoAs | n (kcal mol−1) | ΔH (cal mol−1 K−1) | ΔS (kcal mol−1) | ΔG | Kd |
|---|---|---|---|---|---|
| 14:0 | 1.0±0.0 | −11.6±1.4 | −4.6 | −10.2±0.2 | 38.7±4.5 |
| 16:0 | 1.0±0.1 | −13.7±2.4 | −11.5 | −10.2±0.0 | 35.9±8.4 |
| 18:0 | 1.0±0.0 | −12.0±2.5 | −6.6 | −10.0±0.3 | 60.6±2.4 |
| 18:1 | 1.9±0.1 | −6.7±3.8 | 11.4 | −10.2±0.1 | 45.3±4.3 |
| 18:2 | 1.1±0.0 | −12.3±1.4 | −6.7 | −10.2±0.2 | 36.4±4.9 |
| 18:3 | 1.1±0.0 | −12.1±1.3 | −7.7 | −9.7±0.1 | 84.1±2.9 |