Table 2. Thermodynamic parameters for acyl-CoA binding to rAtACBP4.
ITC experiments were carried out at 30°C in 10 mM sodium phosphate buffer (pH 7.0). The values are the means of three experiments±S.D.
| Acyl-CoAs | n (kcal mol−1) | ΔH (cal mol−1 K−1) | ΔS (kcal mol−1) | ΔG | Kd |
|---|---|---|---|---|---|
| 14:0 | 1.0±0.0 | −53.5±6.9 | −157.5 | −5.8±0.1 | 65.3±22.6 |
| 16:0 | 1.0±0.0 | −77.4±2.5 | −235.5 | −6.1±0.2 | 23.5±27.1 |
| 18:0 | 1.1±0.1 | −8.9±1.0 | −0.6 | −8.7±1.0 | 2.7±1.6 |
| 18:1 | 2.1±0.0 | −6.9±0.1 | −4.3 | −5.6±0.0 | 95.2±0.0 |
| 18:2 | 1.0±0.0 | −73.7±2.6 | −224.5 | −5.8±0.2 | 73.8±0.3 |
| 18:3 | 1.0±0.0 | −96.2±5.3 | −300.5 | −5.1±0.0 | 189.0±0.5 |