. 2014 Dec 12;12:350. doi: 10.1186/s12967-014-0350-7

Table 2.

Analytical details of gene primers for real-time PCR analysis

		Sequence	GenBank, accession #	Length (bp)	Temp (°C)	Efficiency (%)	R ²
CAT-G	Forward	TGACTGACTCTTCTTCTC	NM_001911.2	91	55	108.7	0.997
CAT-G	Reverse	AGGAATTGGTTATTTATACTCT	NM_001911.2	91	55	108.7	0.997
CAT-B	Forward	CTGTGGCAGCATGTGTGG	NM_001908.3	115	60	107.5	0.998
CAT-B	Reverse	GCACCCTACATGGGATTCAT	NM_001908.3	115	60	107.5	0.998
CAT-L	Forward	GGGAGGGCAGTTGAGGAC	NM_001912.4	111	64.5	98.5	0.995
CAT-L	Reverse	GCAAGGATGAGTGTAGGATTCA	NM_001912.4	111	64.5	98.5	0.995
CAT-K	Forward	GCCAGACAACAGATTTCCATC	NM_000396	75	60	108.1	0.999
CAT-K	Reverse	CAGAGCAAAGCTCACCACAG	NM_000396	75	60	108.1	0.999
CAT-S	Forward	GCTGAGGCACGAGATTCC	NM_004079	78	60	103.8	0.996
CAT-S	Reverse	AGTCTCCACTCTGTCATCCA	NM_004079	78	60	103.8	0.996
CYS-B	Forward	GAGTCCCCTCGCCAGATT	NM_000100.2	149	60	104	0.994
CYS-B	Reverse	AACACAGGGAACTTCTTGTTTTCT	NM_000100.2	149	60	104	0.994
CYS-C	Forward	AGGAGACAGACAGAGAAG	NM_000099.2	84	58	95.2	0.998
CYS-C	Reverse	TATGAGAAGCAAGAAGGAA	NM_000099.2	84	58	95.2	0.998
SRPA3	Forward	ACTCCAGACAGACGGCTTTG	NM_001085.4	73	60	100.5	0.995
SRPA3	Reverse	ATTCTCTCCATTCTCAACTCTGC	NM_001085.4	73	60	100.5	0.995
YWHAZ	Forward	ATGCAACCAACACATCCTATC	NM_00113572	178	60	95.3	0.997
YWHAZ	Reverse	GCATTATTAGCGTGCTGTCTT	NM_00113572	178	60	95.3	0.997
PPIA	Forward	CTTGGGCCGCGTCTCCTTCG	NM_021130	285	60	103.4	0.998
PPIA	Reverse	TTGGGAACCGTTTGTGTTTGGGGC	NM_021130	285	60	103.4	0.998
RPL13A	Forward	CGCCCTACGACAAGAAAAAG	NM_012423	206	60	104.6	0.999
RPL13A	Reverse	CCGTAGCCTCATGAGCTGTT	NM_012423	206	60	104.6	0.999