Table 2.
IC 50 values related to exposure to Ag ENMs for Relative growth activity (RGA) and Plating efficiency (PE) assays in A549 cells (Time of exposure 2, 24, 48 h)
| Ag ENMs 50 nm | Ag ENMs 80 nm | Ag ENM 200 nm | ||
|---|---|---|---|---|
| Relative growth activity | ||||
| [μg/cm2] | T = 2 h | 16.8 ± 1.71 | 24.42 ± 1.82 | 23.68 ± 1.51 |
| T = 24 h | 23.68 ± 11.42 | 37.53 ± 17.58 | 36.37 ± 12.84 | |
| T = 48 h | 15.94 ± 1.33 | 26.09 ± 11.63 | 21.71 ± 7.41 | |
| [ENMs × 1011/cm2] | T = 2 h | 6.57 ± 0.6 | 2.51 ± 0.19 | 0.46 ± 0.03 |
| T = 24 h | 9.26 ± 4.47 | 3.86 ± 1.81 | 0.75 ± 0.25 | |
| T = 48 h | 6.23 ± 0.52 | 2.69 ± 1.2 | 0.42 ± 0.14 | |
| [ENMs cm2/cm2] | T = 2 h | 2.3 ± 0.23 | 1.85 ± 0.13 | 0.61 ± 0.53 |
| T = 24 h | 3.24 ± 1.33 | 2.84 ± 1.33 | 1.41 ± 0.5 | |
| T = 48 h | 2.18 ± 0.18 | 1.97 ± 0.88 | 0.84 ± 0.29 | |
| Plating efficiency | ||||
| [μg/cm2] | T = 2 h | 35.58 ± 13.7 | - | - |
| T = 24 h | 31.65 ± 15.81 | 31.65 ± 15.81 | - | |
| T = 48 h | 17.83 ± 4.5 | 14.25 ± 1.94 | - | |
| [ENMs × 1011/cm2] | T = 2 h | 13.91 ± 5.35 | - | - |
| T = 24 h | 12.37 ± 6.18 | 3.66 ± 0.41 | - | |
| T = 48 h | 6.97 ± 1.77 | 1.48 ± 0.2 | - | |
| [ENMs cm2/cm2] | T = 2 h | 4.87 ± 1.89 | - | - |
| T = 24 h | 4.37 ± 2.17 | 2.69 ± 0.3 | - | |
| T = 48 h | 2.44 ± 0.62 | 1.08 ± 0.15 | - |
-IC50 could not be estimated. IC50 values were calculated for 3 different concentration characterization units: number of ENMs (ENMs × 1011/cm2), surface area of ENMs (ENMs cm2/cm2) and mass of ENMs (μg/cm2) per exposure surface.