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. 2014 Dec 20;12:352. doi: 10.1186/s12967-014-0352-5

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© Zhu et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purification of H5N1-specific Nanobodies. (A)Three Nanobodies encoded by different sequence were purified by immobilized metal affinity chromatography (IMAC) using a His-Select matrix. These Nanobodies were detected by coomassie brilliant blue stained SDS-PAGE. (B) Isoelectric point, molecular weight and output of three Nanobodies.