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. 2014 Dec 20;12:352. doi: 10.1186/s12967-014-0352-5

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© Zhu et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of H5N1 virus by directional sandwich ELISA and comparison with the conventionally undirectional ELISA. (A) Serial concentrations (0, 10, 50, 100, 500, 1000, 5000 ng/mL) of H5N1 antigen were used for detection. The linear relationship in the range from 50 to 1000 ng/mL. The linear equation was calculated as Y = 0.0006 X + 0.1640 with an acceptable correlation coefficient of 0.9947 (R²). The data represent mean ± standard deviation from triplicate measurements. (B) The concentration of H5N1 from 0 to 1000 ng/mL to test directional and undirectional sandwich ELISA system. Error bars are standard deviations of three measurements.