Table 1.

Solutions

Components	Pipette				Bath
	a	b	c	d	A	B	C	D	E
NaCl					110	110	55		15
CsCl					10	10	10
CaCl2	1	1	1	1	1.5	1.5	1.5	1.5	1.5
MgCl2					1	1	1	1	1
TEA-Cl	10	10	10	10
TMA-Cl							55	120	105
EGTA	10	10	10	10
HEPES	10	50	50	50	10	10	10	10	10
NaHCO3		8	25	25		25	25	10	10
Cs-Gluconate	125	105	105	90
Cs-HCO3		17
TMA-HCO3								15	15
Na-Gluconate	10			15	25
ATP-Mg	1	1	1	1
ATP-Na2	1	1
Glucose					15	15	15	15	15

Bicarbonate-containing solutions were bubbled with 5% CO₂ and 95% O₂. All solutions were pH 7.4. Glucose was included in the bath solutions to adjust the osmolality to approximately 300 mmol/Kg. The solution osmolality was determined with an osmometer (Model 5520, Vapro® vapor pressure osmometer, Wescor Inc., Logan, UT, USA).