Table 1.
Validation of MS-MLPA results for ATM, MLH1 and FHIT
| Gene | Method | Primer sequence/polyclonal antibody | No. samples examined | Overall concordance (%) |
|---|---|---|---|---|
|
ATM |
Pyrosequencing CpG analysis |
Fw: 5′-AGAAGTGGGAGTTGGGTAGTT-3′ |
77/78 |
73% |
| Rv: 5′-biotinCTCCCCCCCCCTACCACTACACTC-3′ | ||||
| Seq: 5′-AGGAGGAGAGAGGAGT-3′ | ||||
|
MLH1 |
Pyrosequencing CpG analysis |
Fw: 5′-biotinGGGAGGTAAGTTTAAGTGGAATAT-3′ |
72/78 |
79% |
| Rv: 5′-CCAATCCCCACCCTAAAACCCTC-3′ | ||||
| Seq: 5′-CTAAACTCCCAAATAATAACCT-3′ | ||||
| FHIT | Immunohistochemistry | Rabbit polyclonal anti-FHIT; clone PA1-37690; Thermo Scientific Pierce; working dilution: 1/200 | 57/78 | 84% |
Abbreviations: Fw Forward Primer, Rv Reverse primer, Seq sequence analyzed.