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Western blot study for phosphorylation of AMPK and ACC. Phosphorylation of AMPK and ACC in skeletal muscles. Antibody for phosphorylated AMPK, AMPK, ACC, or phosphorylated ACC was used. P-AMPKα (Thr172): phosphorylated AMPK at Thr172, AMPK: total AMPK. P-ACC (Ser79): phosphorylated ACC at Ser79. ACC: total ACC. Wt: wild type mice, A(−/−): AMPD1 deficient homozygote mice. CD: fed with normal chow diet, HFD: after high fat diet challenge.
