Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 12;14:681. doi: 10.1186/s12879-014-0681-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Huang et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Cloning and over-expression of PlyAB1. (A) Cloning of plyAB1 into pET28a: 1) recombinant plasmid pET28a-plyAB1, 2) pET28a-plyAB1 digested with BamHI and Xhol, 3) PCR amplification of plyAB1. (B) The purified PlyAB1 protein after Ni⁺ affinity chromatography and filtration.