Fig. 2.

Quinol oxidase activities of solubilized membranes. Strains were grown under aerobic conditions in 250 ml of TY medium in 500 ml Erlenmeyer flasks, and solubilized membranes were prepared as described in Methods. The addition of KCN depleted activity in all strains. Reaction mixtures with no substrate (quinol) and no membranes were used as separate negative controls (data not shown) that gave no apparent quinol oxidase activity. Mean±sd values were calculated from measured activities from three separate experiments and cultures.