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. 2014 Sep 3;64(1):266–278. doi: 10.2337/db14-0215

Table 2.

Univariate analysis of risk factors versus further worsening retinopathy, new onset of nephropathy, or new onset of neuropathy during EDIC study#

Characteristics Three-step or more ETDRS scale progression from DCCT closeout through EDIC study years 13–16
Development of microalbuminuria or worse (AER ≥ 40 mg/24 h) from DCCT closeout through EDIC study years 15–16
Development of confirmed clinical neuropathy or abnormal autonomic function from DCCT closeout to EDIC study years 13–14 or 16–17*
Event
(N = 91) Nonevent
(N = 122) P value Event
(N = 50) Nonevent
(N = 145) P value Event
(N = 74) Nonevent
(N = 91) P value
Demographic
 Age at EDIC study baseline (years) 33.6 ± 7.0 34.3 ± 6.6 0.52 32.6 ± 6.9 34.5 ± 6.6 0.09 34.7 ± 6.3 32.5 ± 6.5 0.04
 Women (%) 52.8 43.3 0.18 58.0 44.8 0.11 44.6 48.4 0.63
 Diabetes duration at EDIC study baseline (years) 10.5 ± 4.4 11.0 ± 5.0 0.64 10.1 ± 3.9 10.7 ± 4.8 0.70 10.8 ± 4.6 10.4 ± 4.7 0.52
 DCCT treatment group intensives (%) 44.0 65.5 0.002 48.0 60.7 0.12 56.8 56.0 0.93
Glycemia control
 DCCT mean A1C
  % 8.7 ± 1.7 7.5 ± 1.3 <0.0001 8.8 ± 1.6 7.7 ± 1.4 <0.0001 8.3 ± 1.7 7.7 ± 1.3 0.04
  mmol/mol 72 ± 18.6 58 ± 14.2 73 ± 17.5 61 ± 15.3 67 ± 18.6 61 ± 14.2
 EDIC study mean A1C up to EDIC study year 16
  % 8.6 ± 1.2 7.6 ± 0.9 <0.0001 8.9 ± 1.2 7.7 ± 1.0 <0.0001 8.3 ± 1.3 7.7 ± 0.9 0.004
  mmol/mol 70 ± 13.1 60 ± 9.8 74 ± 13.1 61 ± 10.9 67 ± 14.2 61 ± 9.8
Medical at EDIC study baseline
 Mean blood pressure (mmHg) 90.2 ± 10 88.5 ± 7.9 0.23 90.9 ± 8.7 88.4 ± 8.2 0.10 89.9 ± 7.4 87.9 ± 8.7 0.19
 Hypertension (%) 8.8 1.6 0.017 8.0 3.5 0.19 2.7 5.5 0.38
  Triglycerides (mg/dL) 81.3 ± 40 77.6 ± 44 0.39 82.9 ± 42 74.5 ± 40 0.21 86.3 ± 57 75.8 ± 34 0.63
  HDL cholesterol (mg/dL) 52.9 ± 13 50.3 ± 13 0.15 52.1 ± 12 51.5 ± 13 0.74 50.8 ± 15 50.6 ± 11 0.67
  LDL cholesterol (mg/dL) 113 ± 28 108 ± 28 0.16 114 ± 27 108 ± 28 0.08 119 ± 31 106 ± 27 0.004
  Overweight (%) 55.0 54.1 0.90 62.0 53.1 0.28 60.8 49.5 0.15
  Smoker (%) 28.6 18.9 0.10 26.0 18.6 0.27 29.7 18.7 0.10
Retinopathy at EDIC study baseline (%)
 No retinopathy (10/10) 30.8 27.9 20.0 34.5 21.6 35.2
 Microaneurysm only (20/<20) 30.8 41.0 0.22 34.0 39.3 0.008 40.5 42.9 0.01
 Mild NPDR (35/<35) 20.0 24.6 30.0 17.9 24.3 15.4
 Moderate NPDR or worse (43/≤43+) 18.9 6.6 16.0 8.3 13.5 6.6
Renal at EDIC study baseline
 AER (mg/24 h) 10.1 7.2 0.0002 10.1 7.2 0.0009 101.1 7.2 0.007
 Median (interquartiles) (6, 20) (4, 12) (7, 17) (4, 10) (6, 20) (6, 12)
 AER ≥40 (%) 14.3 4.1 0.008 0 ∼ 0 ∼ n/a 16.2 2.2 0.001
Neuropathy at EDIC study baseline (%)
 Confirmed clinical neuropathy 12.2 6.6 0.15 12.0 7.6 0.35 0 0 n/a
 Abnormal autonomic function 15.1 2.8 0.003 9.1 5.7 0.44 0 0 n/a
Skin collagens at EDIC study baseline
 FUR (pmol/mg) 878 ± 251 686 ± 179 <0.0001 875 ± 236 706 ± 194 <0.0001 821 ± 233 691 ± 212 0.0001
 CML (pmol/mg) 580 ± 138 503 ± 117 <0.0001 569 ± 125 513 ± 130 0.009 571 ± 119 502 ± 130 <0.0001
 Pentosidine (pmol/mg) 27.7 ± 7.6 24.6 ± 6.9 0.002 27.5 ± 8.0 24.8 ± 6.8 0.02 27.1 ± 6.9 24.0 ± 6.7 0.005
 Fluorescence (pmol/mg) 193 ± 43 183 ± 52 0.01 194 ± 42 181 ± 51 0.02 192 ± 46 175 ± 40 0.007
 Acid-soluble collagen (%) 0.54 ± 0.4 0.56 ± 0.3 0.03 0.54 ± 0.4 0.57 ± 0.3 0.11 0.50 ± 0.2 0.57 ± 0.3 0.10
 Pepsin-soluble collagen (%) 6.0 ± 3.5 7.2 ± 3.0 0.0001 6.1 ± 2.7 7.2 ± 3.4 0.02 6.0 ± 2.4 7.3 ± 3.5 0.02
 GSPNE (nmoles/mg) 2.8 ± 0.7 2.3 ± 0.5 <0.0001 2.7 ± 0.5 2.4 ± 0.6 0.0001 2.7 ± 0.7 2.3 ± 0.6 <0.0001
 CEL (pmol/mg) 164 ± 120 134 ± 96 0.06 147 ± 83 144 ± 116 0.14 161 ± 115 135 ± 94 0.09
 G-H1 (pmol/mg) 66 ± 42 65 ± 27 0.40 74 ± 47 64 ± 29 0.42 62 ± 36 62 ± 26 0.55
 MG-H1 (nmol/mg) 0.91 ± 0.5 0.73 ± 0.4 0.004 0.90 ± 0.6 0.76 ± 0.4 0.10 0.92 ± 0.6 0.68 ± 0.3 0.006

Data are mean ± SD, unless otherwise indicated.

#Analyses only included subjects who were at risk for an outcome during the EDIC study, with those subjects with a pre-existing outcome during the DCCT being excluded. The retinopathy analysis excluded those who underwent scatter photocoagulation during the DCCT (n = 2). The nephropathy analyses excluded those who had AER >40 mg/24 h at DCCT closeout (n = 20). The neuropathy analysis excluded those who had confirmed clinical neuropathy or abnormal autonomic neuropathy (n = 30) at DCCT closeout. NPDR, nonproliferative diabetic retinopathy.

*Confirmed clinical neuropathy at EDIC study years 13–14 or abnormal autonomic function at EDIC study years 13–14 or 16–17.

†Hypertension was defined as systolic blood pressure ≥140 mmHg or diastolic blood pressure ≥90 mmHg.

‡Overweight was defined as BMI ≥25 kg/m2.