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Melting curves of saffron and its adulterants. Saffron, 7 adulterants, and 1 : 1 mix of authentic saffron with adulterants were amplified in triplicate by the primer pair psbAF/trnHR and performed melting curve analysis by a real-time PCR system. Mixture means mix of saffron with the corresponding adulterant in one PCR reaction.
