Table 4.
Extractability rate of the extraction H2O/CHCl3/MeOH 30/30/40 (v/v/v), repeatability, and reproducibility expressed in terms of the variation (RSD %) of contents. The repeatability and reproducibility of the HPLC-DAD method are expressed in terms of the variation (RSD %) of retention times (RT) and areas.
| Metabolitesa | ||||||||
|---|---|---|---|---|---|---|---|---|
| 5-CQA | 3,5-diCQA | DHLc | Lc | DHdLc | dLc | DHLp | Lp | |
| Extraction | ||||||||
| Extractability rate % | ||||||||
| Root | 94 | 76 | 79 | 82 | 85 | 86 | 84 | 85 |
| Flour | 82 | 82 | 85 | 84 | 85 | 84 | 85 | 85 |
| Roasted grains | 86 | 85 | 88 | 89 | 89 | 87 | 89 | 89 |
| Repeatability RSD % | ||||||||
| Root | ±4 | ±6 | ±2 | ±2 | ±7 | ±9 | ±6 | ±8 |
| Flour | ±6 | ±5 | ±9 | ±7 | ±6 | ±7 | ±5 | ±7 |
| Roasted grains | ±4 | ±4 | ±2 | ±10 | ±7 | ±5 | ±10 | ±4 |
| Reproducibility RSD % | ||||||||
| Root | ±6 | ±6 | ±5 | ±4 | ±9 | ±11 | ±8 | ±8 |
| Flour | ±5 | ±6 | ±7 | ±10 | ±7 | ±9 | ±7 | ±11 |
| Roasted grains | ±6 | ±6 | ±9 | ±8 | ±11 | ±5 | ±12 | ±6 |
|
| ||||||||
| HPLC-DAD analysis | ||||||||
| Repeatability | ||||||||
| RT RSD % | ||||||||
| Root | ±0 | ±0.6 | ±0 | ±0 | ±0.3 | ±0.9 | ±0.6 | ±0.6 |
| Flour | ±0.4 | ±0.6 | ±0.4 | ±0.4 | ±0.5 | ±0.9 | ±0.4 | ±0.3 |
| Roasted grains | ±0.3 | ±0.6 | ±0.3 | ±0.3 | ±0.6 | ±0.8 | ±0.6 | ±0.7 |
| Areas RSD % | ||||||||
| Root | ±5.1 | ±7.2 | ±5.7 | ±3.5 | ±9.7 | ±14.3 | ±11.3 | ±4.1 |
| Flour | ±1.3 | ±1.6 | ±3.2 | ±2.9 | ±6.6 | ±12 | ±13.2 | ±6.6 |
| Roasted grains | ±2.5 | ±4.1 | ±7.7 | ±1.1 | ±6.1 | ±3.3 | ±1.3 | ±1.1 |
| Reproducibility | ||||||||
| RT RSD % | ||||||||
| Root | ±0.6 | ±1.5 | ±1.0 | ±1.0 | ±1.0 | ±0.6 | ±1.5 | ±1.0 |
| Flour | ±0.6 | ±0.6 | ±0 | ±0.6 | ±0.6 | ±1 | ±1.2 | ±0.6 |
| Roasted grains | ±0.6 | ±1.0 | ±0 | ±1.0 | ±1.2 | ±0.6 | ±1.2 | ±1.5 |
| Areas RSD % | ||||||||
| Root | ±11.5 | ±7.7 | ±2.7 | ±11.9 | ±8.3 | ±6.4 | ±10.5 | ±10.2 |
| Flour | ±5.4 | ±3.6 | ±5.5 | ±3.6 | ±3.8 | ±9.5 | ±6.2 | ±4.9 |
| Roasted grains | ±4.4 | ±5.8 | ±8.8 | ±3.3 | ±6.7 | ±8.4 | ±5.1 | ±1.8 |
a5-CQA: 5-mono-O-caffeoylquinic acid; 3,5-diCQA: 3,5-di-O-caffeoylquinic acid; DHLc: 11(S),13-dihydrolactucin; Lc: lactucin; DHdLc: 11(S),13-dihydro-8-deoxylactucin; dLc: 8-deoxylactucin; DHLp: 11(S),13-dihydrolactucopicrin; Lp: lactucopicrin.
RSD: respective standard deviations.