Table 2. Statistics for de novo assembly of the L. santarosai serovar Shermani strain LT821 (ATCC number 43286) genome.
| Sequencing statistics for the genome mapped reads | ||||||
|---|---|---|---|---|---|---|
| Technology | Number of reads | Coverage | Mean read length (bp) | |||
| Illumina 2×75 bp paired-end (500 bpa) | 7 987 144 | CI: 142X; CII: 143X | 75 | |||
| Illumina 2×75 bp mate-pair (3000 bpa) | 27 550 738 | CI: 561X; CII: 548X | 100 | |||
| Roche 454 | 597 201 | CI: 60X; CII: 60X | 410 | |||
| PacBio (filtered subreads) |
40 203 |
CI: 4.65X; CII:4.17X |
3514 |
|||
|
Assembly statistics | ||||||
|
Dataset |
Number of scaffolds (>1 kb) |
Minimun length (bp) |
Maximun length (bp) |
Average (bp) |
N50 (bp) |
Total bases |
| Illumina (paired-end+mate-pair) | 79 | 1055 | 316 426 | 49 532.05 | 101 468 | 3 972 582 |
| Roche 454 | 243 | 101 | 81 757 | 15 956.01 | 26 460 | 3 877 310 |
| PacBio (HGAP) | 13 | 8973 | 20 75 818 | 30 9448.2 | 2 075 818 | 4 022 826 |
| Illumina (paired-end+mate-pair)+PacBio (AHA) | 66 | 169 | 758 047 | 60 811.83 | 366 311 | 4 013 581 |
| Roche 454+PacBio (AHA) | 41 | 1072 | 519 368 | 98 637.02 | 238 493 | 4 044 118 |
Abbreviations: bp, base pair; CI, chromosome I; CII, chromosome II; kb, kilobase.
a
library size.