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Segregation of two single copy STB reporter plasmids. Two single copy STB reporter plasmids cohabiting a nucleus are distinguished by tagging one with green fluorescence [(GFPLacI)-LacO] and the other with red fluorescence [(TetR-RFP)-TetO). Under a functional Rep-STB system, the red and green plasmid sisters segregate most of the time in a one-to-one fashion to yield individual cells containing one red plasmid and one green plasmid (53),
