Skip to main content
. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Free Radic Biol Med. 2013 Sep 27;65:1310–1314. doi: 10.1016/j.freeradbiomed.2013.09.017

Figure 4.

Figure 4

The dynamics of generation of luminescence from L-012 probe during deoxygenation and reoxygenation. (a–d) L-012 (100 μM) was incubated in aqueous solution of phosphate buffer (50 mM, pH 7.4) containing dtpa (100 μM) and H2O2 (50 μM) at 37°C. Where indicated, HRP (0.1 U/ml), SOD (50 μg/ml) or CAT (3 kU/ml) were added during reaction monitoring. In (d), sample was deoxygenated by passing argon gas through the solution before and during the reaction. In (c) and (d), Ar and O2 denote time points at which purging of argon gas and oxygen gas was started.