Figure 1.

Adult human neural crest-derived stem cells derived from the inferior turbinate (ITSCs) are able to efficiently differentiate into the neural lineage. (A): High-cell-density monolayer was cultivated under exposure to a neuronal induction medium. (B): At 7 days after induction, most cells exhibited a neuronal morphology. (C): By differentiation day 24, cells had built up a dense neuronal network. (D): Immunocytochemical staining showed coexpression of βIII-tubulin and neurofilament (NF200) and (E) βIII-tubulin and Map2 at day 24 of differentiation. (F): Quantification of immunocytochemical analyses 24 days after induction; βIII-tubulin: 100% of analyzed cells; neuronal morphology: 70.7 ± 5.1% of βIII-tubulin-positive cells, NF200: 39% ± 10.3% of ITSCs showing neuronal morphology and expression of βIII-tubulin. Abbreviation: DAI, days after induction.