Overexpression promoted the neuronal differentiation of hippocampal radial glial cells. Compared with the mock transfected cell and LV4-NC groups, the Runx1t1 overexpression group showed more MAP-2-positive cells, with more abundant and longer processes (A–C). Scale bar = 50 μm. The number of MAP-2-positive cells significantly differed between the mock transfected cell/LV4-NC groups and the Runx1t1 overexpression group (D). ∗, p < .05. Abbreviations: MAP-2, microtubule-associated protein 2; NC, negative control.