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. 2014 Dec 17;10:77–88. doi: 10.2147/IJN.S71700

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© 2015 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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LOD detection for six combinations of MMSs coupled with H8 or Pab and QDs conjugated with H8, Mab, or mabc.

Notes: Differences in MTB detection capability between the combinations were evident for a range of MTB concentrations in the test. Combinations of MMS-H8+QD-mAb and MMS-Pab+QD-mAbc produced a strong signal for only 10⁷ CFU/mL H₃₇R_v cells, indicating relatively poor MTB detection capability. Combinations of MMS-H8+QD-mAbc and MMS-Pab+QD-mAb exhibited a higher but decreasing fluorescence intensity of H₃₇R_v for 10⁵ CFU/mL or 10⁶ CFU/mL, showing improved MTB detection capability. Combinations of MMS-Pab+QD-H8 and MMS-H8+QD-H8 yield a significantly higher signal for 10³ CFU/mL H₃₇R_v and thus demonstrated the best MTB detection capability.

Abbreviations: LOD, limit of detection; MMSs, magnetic microspheres; QDs, quantum dots; Pab, a rabbit polyclonal antibody against MTB; mAb, a murine monoclonal antibody against MTB; mAbc, a murine monoclonal antibody against MTB heat shock protein 65 (HSP65); CFU, colony-forming unit; mAbc, .