Figure 7.
Apoptosis assay of SCs in each group by flow cytometry and CCK-8 assay.
Notes: (A) Culture-plate group; (B) nonmagnetic membrane group; (C) magnetic membrane group; (D) magnetic membrane + MF group (0.5 mT); (E) magnetic membrane + MF group (1.0 mT); (F) nonmagnetic membrane + MF group (2.0 mT); (G) magnetic membrane + MF group (2.0 mT); (H) nonmagnetic membrane + MF group (5.0 mT); (I) magnetic membrane + MF group (5.0 mT); (J) nonmagnetic membrane + MF group (10.0 mT); (K) magnetic membrane + MF group (10.0 mT). (L) The percentage of apoptotic cells in each group was obtained by averaging the results of five flow-cytometry assays for each group. (M) The CCK-8 values of viable cells in each group were obtained. All data are expressed as means ± standard error of mean. *P<0.05; **P<0.01, one-way ANOVA when compared with culture-plate group.
Abbreviations: SCs, Schwann cells; MF, magnetic field; CCK, Cell Counting Kit; ANOVA, analysis of variance; PI, propidium iodide; h, hours; NS, not significant.
