Figure 7.

Effects of MSNs on the activation of the NF-κB signaling pathway.

Notes: NRK-52E cells were treated with the indicated concentrations of MSNs for 5 min (A),15 min (B), and 30 min (E); or treated with 400 εg/mL MSNs for the indicated time (C, D and F). The proteins (20 εg) of the total lysates and the nuclear (15 εg) and cytoplasm proteins (15 εg) extracted from NRK-52E cells were subjected to 10% SDS-PAGE followed by Western blot with the indicated antibodies. NRK-52E cells treated with 2 εg/mL LPS served as the positive control. Histone H1.4 and GAPDH were used as the loading controls for the nuclear and cytoplasmic proteins, respectively. The blots were then quantified by densitometry. TNF-α, IL-1β, and IL-6 production was assessed using ELISA assay (E and F). The data are presented as the means ± standard error of the mean from three independent experiments. (*P<0.05, **P<0.01, and ***P<0.001 versus con, ANOVA).

Abbreviations: MSNs, mesoporous silica nanoparticles; NF-κB, nuclear factor kappa B; NRK, normal rat kidney; min, minutes; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; con, control; LPS, Lipopolysaccharides.