Skip to main content
. 2014 Sep 1;24(2):371–382. doi: 10.1093/hmg/ddu449

Figure 1.

Figure 1.

Identification of endogenous modulators of γ-secretase and Aβ production. (A) Analysis by LC-MS/MS of the protein components of the highly purified γ-secretase complex. (B and C) siRNA knockdown of TSPAN6, RTN4 and APMAP affects APP-CTFs in HeLa cells (B) and HEK cells overexpressing APP bearing the Swedish mutation that causes early-onset familial Alzheimer's disease (HEK-APPSwe; C). Biological duplicates are shown for each siRNA condition. (D) Correlation between APP-CTFs levels and Aβ40 production in HEK-APPSwe cells with reduced TSPAN6, RTN4 and APMAP expression. APP-CTFs levels were estimated by densitometric analysis, while Aβ40 levels were quantified by ELISA. Student's t-test was applied for statistical analysis; significance is shown as mean ± SD, *P < 0.05; **P < 0.01; Aβ40: n = 6/group; APP-CTFs: n = 4/Scramble, TSPAN6and APMAP groups; n = 3/RTN4 group. β-Actin served as a protein loading control. mAPP-FL and iAPP-FL: mature and immature APP full-length.