Figure 3. DMBA-induction of ovarian Xrcc6 mRNA and protein in lean and obese mice.
Lean and obese mice were dosed with sesame oil (CT) or DMBA (1 mg/kg; ip) for 14 days, followed by RNA or protein isolation. qRT-PCR or Western blotting were performed to quantify Xrcc6 (A) mRNA or (B, C) protein abundance. (A) Xrcc6 was normalized to Gapdh for quantification, and values are reported as fold-change (n = 3) relative to lean CT ovaries (set at 1). (B) Protein abundance was quantified using ImageJ software; Lean control = LC; Lean DMBA = LD; Obese Control = OC, Obese DMBA = OD. Values are expressed as raw data mean ± SE; n=3. Different letters indicate P < 0.05.