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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2014 Oct 22;281(2):203–210. doi: 10.1016/j.taap.2014.10.004

Figure 4. Response of ovarian Prkdc mRNA and protein to DMBA exposure in lean and obese mice.

Figure 4

Lean and obese mice were dosed with sesame oil (CT) or DMBA (1 mg/kg; ip) for 14 days, followed by RNA or protein isolation. qRT-PCR or Western blotting were performed to quantify Prkdc (A) mRNA or (B, C) protein abundance. (A) Prkdc was normalized to Gapdh for quantification, and values are reported as fold-change (n = 3) relative to lean CT ovaries (set at 1). (B) Protein abundance was quantified using ImageJ software; Lean control = LC; Lean DMBA = LD; Obese Control = OC, Obese DMBA = OD. Values are expressed as raw data mean ± SE; n=3. Different letters indicate P < 0.05.