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Published in final edited form as: Cell Signal. 2008 Jan 24;20(5):949–957. doi: 10.1016/j.cellsig.2008.01.009

(A) F9 cells transiently transfected with siRNA sequences targeting OSTM1 (or a control sequence) and expression vectors encoding Fz1 and M50 were treated with or without Wnt3a for 7 hr, then collected and subjected to luciferase assay. * denotes P < 0.05 for the difference between cells transfected with the control siRNA and those transfected with the siRNA targeting mouse OSTM1. (B) Reverse-transcription PCR analysis was used to measure the effect of siRNA treatment on OSTM1 mRNA levels. The expression of cyclophilin A was used as a loading control. (C) F9 cells transiently transfected with siRNA sequences targeting mouse OSTM1 and expression vectors encoding Fz1, M50 and the indicated amounts of human OSTM1 were treated with or without Wnt3a for 7 hr, then collected and subjected to luciferase assay. * denotes P < 0.05 for the difference between cells transfected with the mouse OSTM1 siRNA and those transfected with the siRNA targeting mouse OSTM1 and human OSTM1 cDNA.

(A) F9 cells transiently transfected with siRNA sequences targeting OSTM1 (or a control sequence) and expression vectors encoding Fz1 and M50 were treated with or without Wnt3a for 7 hr, then collected and subjected to luciferase assay. * denotes P < 0.05 for the difference between cells transfected with the control siRNA and those transfected with the siRNA targeting mouse OSTM1. (B) Reverse-transcription PCR analysis was used to measure the effect of siRNA treatment on OSTM1 mRNA levels. The expression of cyclophilin A was used as a loading control. (C) F9 cells transiently transfected with siRNA sequences targeting mouse OSTM1 and expression vectors encoding Fz1, M50 and the indicated amounts of human OSTM1 were treated with or without Wnt3a for 7 hr, then collected and subjected to luciferase assay. * denotes P < 0.05 for the difference between cells transfected with the mouse OSTM1 siRNA and those transfected with the siRNA targeting mouse OSTM1 and human OSTM1 cDNA.