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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Cell Signal. 2008 Jan 24;20(5):949–957. doi: 10.1016/j.cellsig.2008.01.009

Figure 6. OSTM1 regulates β-catenin/Lef1 interaction.

Figure 6

F9 cells transiently transfected with Lef1-HA and either OSTM1, OSTM1ΔC or an empty vector were treated with or without Wnt3a for 3 hours. Cell lysates were collected and subjected to immunoprecipitation with an anti-HA antibody. The amount of endogenous β-catenin pulled down under each condition was measured through Western blot analysis using an anti-β-catenin antibody. Lower panel: Bands from multiple gels were quantified by densitometry. * denotes P < 0.05 for the difference between untreated cells and those treated with Wnt3a.