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. 2014 Dec 23;9(12):e114585. doi: 10.1371/journal.pone.0114585

Figure 5. L1CAM was inversely expressed with miR-503 in osteosarcoma (A) qRT-PCR analysis of L1CAM expression in 30 pair's osteosarcoma tissues and their corresponding adjacent normal tissues.

Figure 5

The expression of L1CAM was normalized to GAPDH. (B) Relative L1CAMexpressionlevels inosteosarcoma tissues and their corresponding adjacent normal tissues.(C) The L1CAM relative mRNA expression levels were determined by qRT-PCR in Human osteosarcoma cell lines (MG-63, U2OS, SOSP-9607, and SAOS-2)) and hFOB. (D) Western blot analysis ofL1CAM expression in Human osteosarcoma cell lines (MG-63, U2OS, SOSP-9607, and SAOS-2)and hFOB. (D) Analysis of correlation of miR-503 and L1CAM expression in osteosarcoma tissues. (Two-tailed Pearson's correlation analysis, r = −0.86; p<0.01, n = 30). Data was presented as log 2 of fold change of osteosarcoma tissues relative to non-tumor adjacent tissues.***p<0.001.