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. 2014 Dec 23;12(12):e1002002. doi: 10.1371/journal.pbio.1002002

Figure 2. Reduction of macrophage numbers in early telogen induces precocious HF growth.

Figure 2

(A) The specific uptake of liposomes by macrophages was analyzed by co-immunofluorescence analysis of F4/80+ cells (red) and the detection of the liposomal PKH67 label (green) in skin sections after the injection of PKH67-liposomes. Arrows indicate double labeling; n = 2. Bar = 20 µm. (B) P44 mice were injected in the backskin for two alternated days with CL-lipo. Samples were collected for analyses at the time indicated in the diagram. (C) Histograms represent the quantification of F4/80+ cells in the backskin after treatment with CL-lipo and Lipo (left). Also shown is the distribution of F4/80+ cells in the backskin after treatment with CL-lipo (right). 10 fields/section/mouse were analyzed; n = 4. (D) Hematoxylin–eosin staining of backskin samples isolated after treatment with CL-lipo and Lipo controls. Bar = 250 µm, n = 4. (E) Histomorphometric analysis of HF stages after macrophages reduction. 100 HFs/mouse were analyzed; n = 4. (F) Appearance of the hair coat at T5 (P69), after shaving and treatment with CL-lipo and Lipo controls at T0 (P44). **p<0.005; ***p<0.0005. Note: n refers to the number of mice, per point per condition. All data used to generate the histograms can be found in Data S1.