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. 2014 Dec 23;9(12):e113978. doi: 10.1371/journal.pone.0113978

Figure 4. LT-IIb and LT-IIc enhance antigen-specific CD8+ T cell expansion after immunization, but with different kinetics.

Figure 4

(A) Representative flow analysis of OVA-specific CD8+ T cells identified by MHC-dextramers 7 days after 1.0 µg HLT adjuvanted immunization. Cells gated on live TCRβ+CD8+ cells. (B) OVA-specific CD8+ T cells from PBMCs after 7, 14, and 28 days post-immunization utilizing 1.0 µg and (C) 0.1 µg of HLT adjuvants. Data shown (n = 8) as a representative of two independent experiments. Statistical analysis: One-way ANOVA with Bonferroni post-test compared to OVA, unless otherwise noted. *P≤0.05; **P≤0.01; ***P≤0.001. Results shown as the arithmetic mean with error bars denoting SEM.