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. 2014 Dec 23;9(12):e114575. doi: 10.1371/journal.pone.0114575

Figure 1. Isolation strategy of single naïve B cells and comparison of the frequencies of naïve, memory switched and unswitched B cells in SS patients and HD.

Figure 1

(A) PBMC from SS patients were surface labeled with fluorochrome-coupled anti-CD19, anti-CD3, anti-IgD and anti-CD27. CD19+CD3− cells were gated and analyzed for IgD and CD27 expression. (B) The frequencies of peripheral naïve (CD3−CD19+CD27−IgD+), memory switched (CD3−CD19+CD27+IgD–) and unswitched (CD3−CD19+CD27+IgD+) B cells among all CD19+ B cells are shown. Differences between patients and HD were statistically significant using the nonparametric Mann-Whitney U test (p value is reported over each graph). Error bars indicate standard error of the mean (SEM) for individual patient or control.