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. 2014 Dec 23;9(12):e115400. doi: 10.1371/journal.pone.0115400

Figure 4. Identification of putative SOX10-response elements in S100B.

Figure 4

(A) Luciferase activities after transfection of putative Schwann cell-related transcription factors into HeLa cells with a reporter construct containing a fragment (−1,000 to +200 bp) of the S100B gene. *P<0.05 versus GFP. (B) Deletion analysis using luciferase-reporter constructs containing a series of deletion fragments of the S100B gene in HeLa cells transfected with SOX10 or control GFP. (C) Comparison of human (Hs), rat (Rn), and mouse (Mm) sequences in three putative SOX motifs in the S100B promoter and mutated sequences (Mut A, Mut B, and Mut C), used in the following mutagenesis analysis. (D) Site-directed mutagenesis analysis using luciferase-reporter constructs containing −334 to +200 bp of the S100B gene with mutations as in Fig. 3C within the three SOX motifs in the cells above. *P<0.05 versus wild-type (WT) with SOX10. All experiments were repeated independently three times with data shown as the mean ± SEM.