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. 2014 Dec 23;9(12):e115648. doi: 10.1371/journal.pone.0115648

Figure 2. Mitragynine suppressed I Kr in hERG-HEK cells.

Figure 2

The effect of Mitragynine was tested in hERG-HEK cells. (A) The time-course and wash out of Mitragynine (n = 4). The insert is the voltage protocol used to elicit hERG. (B) A representative I kr peak tail current traces recorded with Mitragynine (10 µM) treatment. The insert is the voltage protocol used to elicit hERG. (C) The I-V curve of I kr tail currents recorded in hERG-HEK cells prior to and post Mitragynine treatment. *p<0.05. Data represent are the whole cell currents (raw current) uncorrected for the presence of background currents. (D) The steady-state inactivation properties of I Kr in hERG-HEK293 cells. Conditioning pulses between −120 and +20 mV in 10 mV increments for 20 milliseconds were applied after a depolarizing pulse to +20 mV for 900 milliseconds, followed by a common test pulse to +20 mV. The voltage protocol is illustrated in the inset. *p<0.05 (n = 5). The voltage protocol is illustrated in the inset.