Figure 5. Arenavirus phylogenetic relationship inferred by MEGA software based on partial nucleotide sequences of the N gene of 18 NWVs and amplicons generated from PCR positive ticks.

Arenaviruses are listed by name and immediately followed by the GenBank accession number of the strain used in this analysis. Numbers 112, 124, 126, 127, 129, 136 and 149 represent individual ticks with the greatest number of virus copies detected by RT-qPCR. The evolutionary history was inferred using the UPGMA method. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site.