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. 2014 Dec 23;9(12):e115975. doi: 10.1371/journal.pone.0115975

Figure 3. in vitro transphosphorylation of VicR and GcrR by VicK.

Figure 3

A) Phosphorylation of VicR and GcrR by VicK in the presence of MgCl2. For each reaction 1 µM of each of the following proteins were included in the reaction: Lane 1: VicK; Lane 2: ComE; Lane 3: VicR; Lane 4: GcrR; Lane 5: VicK and ComE; Lane 6: VicK and VicR; Lane 7: VicK and GcrR; Lane 8: VicK, ComE and VicR; Lane 9: VicK, ComE and GcrR. B) Phosphorylation of VicR and GcrR by VicK in the presence of MnCl2. For each reaction 1 µM of each of the following proteins were included in the reaction unless otherwise indicated: Lane 1: VicK; Lane 2: VicR; Lane 3: GcrR; Lane 4: VicK and VicR; Lane 5: VicK and GcrR; Lane 6: VicK, GcrR and 0.01 µM VicR; Lane 7: VicK, GcrR and 0.02 µM VicR; Lane 8: VicK, GcrR and 0.04 µM VicR; Lane 9: VicK, GcrR and 1 µM VicR. The gels shown are a representative set of replicate gels run for each experiment.