|
E. coli
|
|
|
| ER2566 |
F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] |
New England Biolabs |
|
S. mutans
|
|
|
| UA159 |
Wild type, serotype c |
ATCC 700610 |
| GMS584 |
UA159-derived, sloR-deficient; EmR
|
[9]
|
| SmuvicK |
UA159-derived, vicK-deficient; EmR
|
[29]
|
| GMS905 |
UA159-derived, with PgcrR:cat integrated; KanR
|
This study |
| GMS906 |
GMS584-derived, with PgcrR:cat integrated; EmR, KanR
|
This study |
| GMS907 |
SmuvicK-derived, with PgcrR:cat integrated; EmR, KanR
|
This study |
|
Plasmids
|
|
|
| pTXB1 |
E. coli expression vector with Mxe intein/chitin binding domain |
New England Biolabs |
| pSG752 |
pTXB1 with vicK; AmpR
|
This study |
| pSG893 |
pTXB1 with vicR; AmpR
|
This study |
| pSG901 |
pTXB1 with gcrR; AmpR
|
This study |
| pSG892 |
pTXB1 with comE; AmpR
|
This study |
| pJL84 |
S. mutans integration vector with mtlA-cat-phnA; KanR
|
[44]
|
| pLM1 |
pJL84 with PgcrR:cat fusion; KanR
|
This study |
