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. 2014 Dec 23;9(12):e115905. doi: 10.1371/journal.pone.0115905

Figure 1. Construct designs involved in characterisation of SbtA transporters.

Figure 1

In all constructs, expression of target proteins was driven by the lac promoter on plasmid pSE2. A. Schematic of typical SbtA constructs shown in Table 4. B. Schematic of typical SbtAB constructs shown in Table 4. In order to generate 7942AnsB, the start codon of sbtB (ATG) in SbtAB7942 construct was replaced with (GGG) to form a SmaI site and a later GTG -valine 41 bp downstream of the start codon was replaced with GTC-valine. In this way, expression of sbtB is completely abolished in 7942AnsB. C. Illustrated location for the c-Myc tag in 7942AMyc. SD, Shine-Dalgarno sequence.