Figure 8. Isolation of SbtA7942 by IMAC using SbtB-HAH6 as binding partner and detected by western blotting.

Gene expression was induced for 2.5 h with 1 mM IPTG. The membrane-enriched protein fractions of E. coli containing the empty pSE2, 7942AMyc and 7942AMBH and 7942B-HAH6 vector were used for IMAC isolation. A total of 40 µg total protein of IMAC elutes per lane was separated by SDS-PAGE and subjected to Western blotting. Proteins were detected with the Anti-c-Myc antibody.