Figure 2. Ethanol-induced expression of BNIP3 and NIX was abolished in primary cultured HIF-1β knockout mouse hepatocytes.

(A) Primary cultured wild type and HIF-1β knockout hepatocytes were treated with ethanol (80 mM) for 6 hours. mRNA was isolated from cultured hepatocytes and real-time RT-PCR was performed as described in the Materials and Methods. Data are presented as means ± SE (n = 4). * p<0.05. One way ANOVA with Scheffé's post hoc test. (B) Cells were treated as in (A), total cell lysates were subjected to western blot analysis. Densitometry was performed and data are presented as a ratio vs HIF-1β, Alb Cre- control (n = 3).