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. 2014 Dec 23;9(12):e115849. doi: 10.1371/journal.pone.0115849

Figure 6. Changes in markers of hepatic inflammation in wild type and hepatocyte-specific HIF-1β knockout mice after Gao-binge treatment.

Figure 6

Age matched male wild type and hepatocyte-specific HIF-1β knockout mice were subjected to Gao-binge treatment. Hepatic mRNA was isolated, and real-time RT-PCR was performed as described in the Materials and Methods (A). Data are presented as means ± SE (n = 3–5). Liver tissue was subjected to immunostaining for neutrophils using an anti-Ly6B antibody and representative images are shown in (B, 40x), and the number of neutrophils were quantified from 10 different fields of each mouse. (C). Data are presented as average number of neutrophils in each filed (means ± SE; n = 4).