Table 7. Virulence genes in B. pseudomallei K96243, 1106a and MSHR668 genomes with metabolic and regulatory functions.
| Gene | Annotated Function | Pathways (KEGG, MetaCyc) or process |
| Metabolism | ||
| BPSL0338 | non-hemolytic phospholipase C (EC 3.1.4.3) | Inositol phosphate metabolism, Glycerophospholipid metabolism, Ether lipid metabolism |
| BPSL0374 | metallo-beta-lactamase superfamily protein | NA |
| BPSL0395 | cytidylyltransferase | various |
| BPSL0413 | lipoate protein ligase B (EC 2.7.7.63) | Lipoic acid metabolism |
| BPSL0634 | oxidoreductase | various |
| BPSL0808 | peptidase; serine protease (EC 3.4.21.-) | various |
| BPSL0908 | phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) | Purine metabolism, One carbon pool by folate, Biosynthesis of secondary metabolites |
| BPSL1103 | endonuclease III (EC 4.2.99.18)# | various |
| BPSL1196 | acetolactate synthase 3 catalytic subunit (EC 2.2.1.6)# | Branched chain amino acid biosynthesis, Butanoate metabolism, C5-branched dibasic acid metabolism, Pantothenate and CoA biosynthesis, Biosynthesis of secondary metabolites |
| BPSL1561 | metallo-beta-lactamase | Hydrolysis of beta-lactam antibiotics |
| BPSL1776 | L-ornithine 5-monooxygenase MbaA/PvdA (EC 1.13.12.-) | Siderophore biosynthesis |
| BPSL1777 | siderophore-related non-ribosomal peptide synthase MbaI | Siderophore biosynthesis |
| BPSL1778 | siderophore related non-ribosomal peptide synthase MbaJ | Siderophore biosynthesis |
| BPSL1876 | phospholipase; phosphoesterase | various |
| BPSL2403 | non-hemolytic phospholipase C (EC 3.1.4.3) | Inositol phosphate metabolism, Glycerophospholipid metabolism, Ether lipid metabolism |
| BPSL2433 | peptidase; Do family protease; serine protease | various |
| BPSL2519 | phosphoserine aminotransferase (EC 2.6.1.52)# | Glycine, serine and threonine metabolism, Methane metabolism, Vitamin B6 metabolism |
| BPSL2672 | epimerase/dehydratase capsule polysaccharide biosynthesis protein | various |
| BPSL2673 | undecaprenyl phosphate N-acetylglucosaminyltransferase; glycoside hydrolase family protein; UDP-D-N-acetylhexosamine:polyprenol phosphate D–N-acetylhexosamine-1-phosphate transferases (EC 2.7.8.-) | various |
| BPSL2674 | NAD-dependent epimerase/dehydratase | various |
| BPSL2675 | glycosyl transferase | various |
| BPSL2676 | glycosyl transferase | various |
| BPSL2677 | O-antigen methyl transferase (EC 2.4.1.-) | various |
| BPSL2678 | glycosyl transferase | various |
| BPSL2679 | NAD-epimerase/dehydratase | various |
| BPSL2680 | O-antigen acetylase WbiA (EC 2.3.1.-) | various |
| BPSL2683 | dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)# | Biosynthesis of secondary metabolites |
| BPSL2684 | dTDP-6-deoxy-D-glucose-3,5 epimerase (EC 5.1.3.13)# | Biosynthesis of secondary metabolites |
| BPSL2685 | glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24)# | Biosynthesis of secondary metabolites |
| BPSL2686 | dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)# | Biosynthesis of secondary metabolites |
| BPSL2687 | diadenosine tetraphosphatase (EC 3.6.1.41)# | Purine metabolism |
| BPSL2688 | 1-acyl-SN-glycerol-3-phosphate acyltransferase; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis (EC 2.3.1.51)# | various |
| BPSL2786 | acetyltransferase | various |
| BPSL2787 | acyl-CoA transferase; 8-amino-7-oxononanoate synthase (EC 2.3.1.47)# | Biotin metabolism |
| BPSL2788 | UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (EC 3.5.1.108)# | various |
| BPSL2789 | capsular polysaccharide biosynthesis fatty acid synthase; type I polyketide synthase WcbR | various |
| BPSL2790 | capsular polysaccharide biosynthesis transmembrane protein; sulfatase (EC 3.1.6.-) | various |
| BPSL2791 | capsular polysaccharide biosynthesis dehydrogenase/reductase; short chain dehydrogenase/reductase family oxidoreductase | various |
| BPSL2792 | capsule polysaccharide biosynthesis/export protein KpsS | various |
| BPSL2793 | D-glycero-d-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.3.82)# | various |
| BPSL2794 | D-glycero-d-manno-heptose 1-phosphate guanosyltransferase (EC 2.7.7.71) | various |
| BPSL2795 | phosphoheptose isomerase (EC 5.3.1.28)# | Lipopolysaccharide biosynthesis |
| BPSL2796 | sugar kinase; D-glycero-D-manno-heptose 7-phosphate kinase; related to galactokinase and mevalonate kinase (EC 2.7.7.70)# | Lipopolysaccharide biosynthesis |
| BPSL2797 | GDP sugar epimerase/dehydratase; GDP-6-deoxy-D-lyxo-4-hexulose reductase (EC 1.1.1.281)# | Fructose and mannose metabolism, Amino sugar and nucleotide sugar metabolism |
| BPSL2798 | capsular polysaccharide biosynthesis protein; NAD-dependent epimerase/dehydratase | various |
| BPSL2799 | capsular polysaccharide biosynthesis protein | various |
| BPSL2800 | glycosyl transferase | various |
| BPSL2801 | capsular polysaccharide biosynthesis protein | various |
| BPSL2802 | capsular polysaccharide biosynthesis protein | various |
| BPSL2803 | glycosyltransferase | various |
| BPSL2808 | capsular polysaccharide glycosyltransferase biosynthesis protein | various |
| BPSL2810 | GDP-mannose pyrophosphorylase; mannose-1-phosphate guanylyltransferase (EC 2.7.7.13/EC 2.7.7.22) | Fructose and mannose metabolism, Amino sugar and nucleotide sugar metabolism, Biosynthesis of secondary metabolites |
| BPSL2818 | phosphoribosylaminoimidazole synthetase (EC 6.3.3.1)# | Purine metabolism, Biosynthesis of secondary metabolites |
| BPSL2825 | hypothetical protein BPSL2825; para-aminobenzoate synthase, component I PabB (EC 2.6.1.85)# | tetrahydrofolate biosynthesis and salvage, superpathway of chorismate metabolism, superpathway of tetrahydrofolate biosynthesis, 4-aminobenzoate biosynthesis |
| BPSL3051 | anthranilate synthase component II (EC 4 1.3.27) | Phenylalanine, tyrosine and tryptophan biosynthesis, Biosynthesis of secondary metabolites |
| BPSL3133 | imidazole glycerol phosphate synthase subunit HisF (EC 4.1.3.−/EC 2.4.2.-)# | Histidine biosynthesis, Purine biosynthesis |
| BPSL3168 | 3-dehydroquinate synthase (EC 4.2.3.4)# | Phenylalanine, tyrosine and tryptophan biosynthesis, Biosynthesis of secondary metabolites |
| BPSS0067 | non-hemolytic phospholipase C (EC 3.1.4.3) | Inositol phosphate metabolism, Glycerophospholipid metabolism, Ether lipid metabolism |
| BPSS0419 | glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33)# | Starch and sucrose metabolism, Amino sugar and nucleotide sugar metabolism, Biosynthesis of secondary metabolites |
| BPSS0420 | CDP-glucose 4,6-dehydratase (EC 4.2.1.45)# | Amino sugar and nucleotide sugar metabolism, Biosynthesis of secondary metabolites |
| BPSS0421 | lipopolysaccharide biosynthesis protein rfbH | Lipopolysaccharide biosynthesis |
| BPSS0422 | aminotransferase | various |
| BPSS0424 | glycosyl transferase group 2 | various |
| BPSS0425 | heptosyltransferase (O-antigen related) | Lipopolysaccharide biosynthesis |
| BPSS0426 | heptosyltransferase (O-antigen related) | Lipopolysaccharide biosynthesis |
| BPSS0427 | O-acetyl transferase; galactoside O-acetyltransferase | Lipopolysaccharide biosynthesis |
| BPSS0428 | glycosyl transferase (O-antigen related) | Lipopolysaccharide biosynthesis |
| BPSS0581 | salicylate biosynthesis isochorismate synthase (EC 5.4.4.2)# | Ubiquinone biosynthesis, Biosynthesis of siderophore group nonribosomal peptides, Biosynthesis of secondary metabolites |
| BPSS0582 | isochorismate-pyruvate lyase (EC 4.2.99.21) | Siderophore biosynthesis |
| BPSS0583 | pyochelin biosynthetic protein PchC (EC 3.1.2.-) | Siderophore biosynthesis |
| BPSS0584 | salicyl-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58)# | Siderophore biosynthesis |
| BPSS0586 | pyochelin synthetase | Siderophore biosynthesis |
| BPSS0587 | pyochelin synthetase | Siderophore biosynthesis |
| BPSS0588 | pyochelin biosynthetic protein | Siderophore biosynthesis |
| BPSS0666 | peptidase; collagenase (EC 3.4.24.3) | Digestion of native collagen |
| BPSS0885 | N-acylhomoserine lactone synthase; autoinducer synthase BpsI (EC 2.3.1.184) | Quorum sensing |
| BPSS0946 | beta-lactamase precursor | Hydrolysis of beta-lactam antibiotics |
| BPSS1180 | N-acylhomoserine lactone synthase; autoinducer synthetase | Quorum sensing |
| BPSS1570 | N-acylhomoserine lactone synthase; autoinducer synthetase BpmI | Quorum sensing |
| BPSS1705 | 3-isopropylmalate dehydrogenase (EC 1.1.1.85)# | Branched chain amino acid biosynthesis, Butanoate metabolism, C5-branched dibasic acid metabolism, Biosynthesis of secondary metabolites |
| BPSS1825 | glycosyltransferase | various |
| BPSS1826 | glycosyltransferase | various |
| BPSS1828 | glycosyltransferase group 1 protein | various |
| BPSS1829 | glycosyltransferase | various |
| BPSS1830 | exopolysaccharide capsular polysaccharide biosynthesis-like tyrosine-protein kinase | capsule biosynthesis |
| BPSS1831 | exopolysaccharide (EPS) capsular polysaccharide biosynthesis related polysaccharide lipoprotein | capsule biosynthesis |
| BPSS1832 | exopolysaccharide (EPS) capsular polysaccharide biosynthesis-like; low molecular weight protein-tyrosine-phosphatase | capsule biosynthesis |
| BPSS1833 | UDP-glucose 6-dehydrogenase 2 (EC 1.1.1.22)# | Pentose and glucuronate interconversions, Ascorbate and aldarate metabolism, Starch and sucrose metabolism, Amino sugar and nucleotide sugar metabolism,Biosynthesis of secondary metabolites |
| BPSS1834 | lipopolysaccharide biosynthesis-like protein; undecaprenyl-phosphate glucose phosphotransferase (EC 2.7.8.31) | NA |
| BPSS1915 | metallo-beta-lactamase | NA |
| BPSS1993 | serine metalloprotease precursor | NA |
| BPSS1997 | class D beta-lactamase | Hydrolysis of beta-lactam antibiotics |
| Regulation | NA | |
| BPSL0812 | TetR family regulatory protein; multidrug efflux pump repressor protein BpeR | NA |
| BPSS0887 | N-acylhomoserine lactone dependent regulatory protein; autoinducer-binding transcriptional regulator BpsR | NA |
| BPSS1176 | N-acyl-homoserine lactone dependent regulatory protein; ATP-dependent transcriptional regulator LuxR | NA |
| BPSS1569 | N-acylhomoserine lactone-dependent regulatory protein; autoinducer-binding transcriptional regulator BmpR | NA |
| BPSL1787 | extracytoplasmic-function sigma-70 factor | NA |
| BPSL1805 | TetR family regulatory protein; multidrug efflux operon transciptional regulator AmrR | NA |
| BPSL2347 | LuxR family transcriptional regulator | NA |
| BPSL2434 | sigma E factor regulatory protein | NA |
| BPSL2435 | sigma E factor negative regulatory protein, RseA family | NA |
| BPSL2866 | oxidative stress regulatory protein OxyR; LysR family transcriptional regulator | NA |
| BPSS0312 | LuxR family transcriptional regulator | NA |
| BPSS0585 | AraC family transcriptional regulator PchR | NA |
| BPSS1391 | AraC family transcriptional regulator | NA |
| BPSS1520 | AraC family transcriptional regulator | NA |
| BPSS1522 | two-component response regulator; LuxR family DNA-binding response regulator | NA |
While K96243 GenBank locus tags are listed, genes are present in all three genomes.
NA: no pathway associated with the enzyme.
Various: enzyme may participate in multiple pathways or annotation too general to identify pathways by EC number.
# Candidate chokepoint.
All of the genes in this table were present in various other B. pseudomallei genomes, as determined by NCBI BLAST against all genomes in GenBank.