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. 2014 Dec 23;9(12):e114766. doi: 10.1371/journal.pone.0114766

Figure 14. Effect of Leukotriene on bleomycin induced cytotoxicity in IMR-32 cells.

Figure 14

(A) Effect of pre-treatment with different doses (10, 50, 100 ng/ml) of leukotriene (LTD4, LTE4) on bleomycin (60 µg/ml) induced cytotoxicity to IMR-32 cells. Cells were pre incubated with leukotriene for 5 hrs and then bleomycin for 24 hrs. MTT assay was performed. (B) Effect of simultaneous treatment with different doses (10, 50, 100 ng/ml) of leukotriene (LTD4, LTE4) on bleomycin (60 µg/ml) induced cytotoxicity to IMR-32 cells. Cells were pre incubated with plain media for 5 hrs and then leukotriene and bleomycin were added and incubated for 24 hrs. MTT assay was performed. All values are expressed as mean ± standard error (n = 6). *P<0.05 when compared to control; #P<0.05 when compared to bleomycin. BLM – Bleomycin, LT - Leukotriene.